GE17-5318-02
Ni Sepharose™ 6Fast Flow
Cytiva 17-5318-02, pack of 100 mL
Sinónimos:
Ni-Sepharose Fast Flow, Nickel Affinity Resin, Nickel Column
About This Item
Productos recomendados
envase
pack of 100 mL
fabricante / nombre comercial
Cytiva 17-5318-02
matriz
6% cross-linked agarose
tamaño de partícula
45-165 μm
diámetro promedio
90 μm
cleaning in place
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacidad
~40 mg binding capacity(histidine-tagged protein)
idoneidad
suitable for bioprocess medium
Descripción general
Ni Sepharose™ 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.
Ni Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity..
Ni Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As A member of the BioProcess media range, Ni Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Características y beneficios
- Designed for scaling up purification of histidine-tagged proteins
- High protein binding capacity and minimal leakage of Ni2? ions
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in convenient prepacked HisPrep™ and HisTrap™ column formats.
Almacenamiento y estabilidad
Nota de análisis
Información legal
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Protocolos
This page shows how to purify histidine-tagged recombinant proteins manually with a syringe using HisTrap FF crude Kit from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from GE Healthcare.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from GE Healthcare.
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