GE17-0407-03
HiTrap® Heparin High Performance
Cytiva 17-0407-03, pack of 5 × 5 mL
Sinónimos:
Heparin HP columns
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About This Item
Código UNSPSC:
23151816
NACRES:
NA.56
ligand
porcine heparin
envase
pack of 5 × 5 mL
fabricante / nombre comercial
Cytiva 17-0407-03
Parámetros
42 psi
lecho tamaño
16 mm × 25 mm
lecho volumen
5 mL
columna D.I.
16 mm
matriz
highly cross-linked 6% agarose
tam. prom. pieza
34 μm
cleaning
5-10
working range
5-10
Descripción general
HiTrap® Heparin HP columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.
Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.
Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.
Características y beneficios
- Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.
- Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.
- Simple operation with a syringe, a pump or a chromatography system such as AKTA design
- Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.
Almacenamiento y estabilidad
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Nota de análisis
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Otras notas
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Información legal
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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