Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

View All Documentation

G5507

Sigma-Aldrich

Galactosyltransferase from bovine milk

lyophilized powder

Sinónimos:

Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número de CAS:
9031-68-9
Comisión internacional de enzimas:
2.4.1.22 (BRENDA, IUBMB)
Número MDL:
MFCD00131122
Código UNSPSC:
12352204
NACRES:
NA.54

formulario

lyophilized powder

Nivel de calidad

200

actividad específica

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

composición

Protein, 35-65% modified Warburg-Christian

impurezas

≤10 % protein α-lactalbumin

temp. de almacenamiento

−20°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Descripción general

Research area: Cell signalling. Galactosyltransferase(GalT) is found in both prokaryotes and eukaryotes.Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.

Aplicación

Galactosyltransferase from bovine milk has been used:
  • in the generation of hyper-galactosylated variant of IgG1
  • in β -1,4-galactosyltransferase assay rice root cell extracts
  • in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Acciones bioquímicas o fisiológicas

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.
Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various disease states.Galactosyltransferase(GalT) functions either as a soluble enzyme or as membrane-bound moieties.

Ligadura / enlace

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Definición de unidad

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Forma física

Lyophilized powder containing Tris, EDTA, and (NH4)2SO4

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Structure and function of beta-1, 4-galactosyltransferase
Qasba PK, et al.
Current Drug Targets, 9(4), 292-309 (2008)
Hierarchical self-assembly and emergent function of densely glycosylated peptide nanofibers
Restuccia A, et al.
Communications Chemistry, 2(1), 53-53 (2019)
In vitro glycoengineering of IgG1 and its effect on Fc receptor binding and ADCC activity
Thomann M, et al.
PLoS ONE, 10(8), e0134949-e0134949 (2015)
A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities.
J A Campbell et al.
The Biochemical journal, 326 ( Pt 3), 929-939 (1997-10-23)
A novel short-root gene encodes a glucosamine-6-phosphate acetyltransferase required for maintaining normal root cell shape in rice
Jiang H, et al.
Plant Physiology, 138(1), 232-242 (2005)
Ver todos los artículos relacionados

Artículos

Glycosyltransferases

Glycosyltransferases were initially considered to be specific for a single glycosyl donor and acceptor, which led to the one enzyme-one linkage concept. Subsequent observations have refuted the theory of absolute enzymatic specificity by describing the transfer of analogs of some nucleoside mono- or diphosphate sugar donors.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico