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F4042

Sigma-Aldrich

Monoclonal ANTI-FLAG® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

Sinónimos:

Anti-ddddk, Anti-dykddddk

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About This Item

Número MDL:
MFCD00282246
Código UNSPSC:
12352203
NACRES:
NA.32

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

M5, monoclonal

formulario

buffered aqueous solution

purificado por

using Protein A

reactividad de especies

all

concentración

2-5 mg/mL

técnicas

western blot (chemiluminescent): 10 μg/mL

isotipo

IgG1

secuencia del inmunógeno

DYKDDDDK

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A

Inmunógeno

FLAG; peptide sequence DYKDDDDK

Aplicación

Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Forma física

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Nota de preparación

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

Otras notas

Antibody is not calcium dependent.

Información legal

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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