F3040
Monoclonal ANTI-FLAG® M1 antibody
clone M1, purified immunoglobulin, buffered aqueous solution
Sinónimos:
Anti-ddddk, Anti-dykddddk
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About This Item
Productos recomendados
origen biológico
(Bioreactor)
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
M1, monoclonal
Formulario
buffered aqueous solution
purificado por
using Protein A
reactividad de especies
all
concentración
2-5 mg/mL
técnicas
western blot: 10 μg/mL
secuencia del inmunógeno
DYKDDDDK
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Descripción general
The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.
Method of purification - Protein A
Method of purification - Protein A
Inmunógeno
FLAG; peptide sequence DYKDDDDK
Aplicación
Monoclonal ANTI-FLAG® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Learn more product details in our FLAG® applications portal.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Learn more product details in our FLAG® applications portal.
Acciones bioquímicas o fisiológicas
Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.
Forma física
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).
Nota de preparación
Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.
Información legal
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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