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CELLMM2

Millipore

FLAG® M Purification Kit

For Mammalian expression systems.

Sinónimos:

Anti-ddddk, Anti-dykddddk

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

Nivel de calidad

200

técnicas

protein extraction: suitable

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Aplicación

The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Learn more product details in our FLAG® application portal.

Características y beneficios

  • Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
  • ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
  • Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Envase

Sufficient for 3-5 uses of 1-ml affinity purification column.

Otras notas

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Información legal

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.SDS
  • SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powderSDS
  • A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solutionSDS
  • C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.SDS

Código de clase de almacenamiento

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Proceedings of the National Academy of Sciences of the United States of America, 111(36), E3785-E3794 (2014-08-27)
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Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein Expression

Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.

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