CWA-1064

Anti-Human CD3ε (APA1/1) ColorWheel^® Dye-Ready mAb

for use with ColorWheel^® Dyes (Required, sold separately)

• Código UNSPSC: 12352203
• NACRES: NA.41
• clon: APA1/1, monoclonal
• application: FACS
• reactividad de especies: human
• técnicas: flow cytometry: suitable

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: APA1/1, monoclonal
• Línea del producto: ColorWheel^®
• Formulario: lyophilized
• mol peso: calculated mol wt 23.15 kDa
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• técnicas: flow cytometry: suitable
• isotipo: IgG1
• secuencia del epítopo: C-terminal half.
• Nº de acceso Protein ID: NP_000724.1
• Nº de acceso UniProt: P07766
• compatibilidad: for use with ColorWheel^® Dyes (Required, sold separately)
• categoría alternativa más sostenible: , Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CD3E(916)

Descripción

Descripción general

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Especificidad

Clone APA1/1 is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD3 epsilon chain (CD3 epsilon). It targets an epitope within the activation motif.

Inmunógeno

SDS denatured CD3 epsilon from human thymus.

Aplicación

Quality Control Testing:

Evaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis (FC): Staining of one million Jurkat cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1064, Anti-Human CD3e (APA1/1) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibilidad

Requires ColorWheel^® Dye (Sold Separately)

Descripción de destino

T-cell surface glycoprotein CD3 epsilon chain (UniProt: P07766; also known as T-cell surface antigen T3/Leu-4 epsilon chain, CD3e) is encoded by the CD3E (also known as T3E) gene (Gene ID: 916) in human. CD3 is a single-pass type I membrane protein that is a part of the TCR-CD3 complex present on T-cells and plays an essential role in adaptive immune response. The TCR-CD3 complex is formed through noncovalent association of TCR with a CD3 signalling apparatus consisting of the  γ, δ, ε and ζ subunits. In addition of this role of signal transduction in T-cell activation, CD3ε plays an essential role in correct T-cell development. It participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3 cytosolic region. CD3ε is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 23-126), a transmembrane protein (aa 127-152), and a cytoplasmic domain (aa 153-207). The CD3 complex performs two major functions. Firstly, CD3 chains are required for intracellular signaling where tyrosines in its immunoreceptor tyrosine-based activation motif (ITAM) domain (aa 178-205) are phosphorylated by Lck. Secondly, the CD3 complex is involved in TCR surface expression. In the endoplasmic reticulum, the TCR heterodimer physically associate with the CD3 complex prior to moving to the Golgi for glycosylation and subsequently translocating to the T-cell surface. Mutations in CD3E gene are known to cause immunodeficiency 18 (IMD18) that is characterized by recurrent infections in infancy or early childhood with variable severity. (Ref.: Dong, D., et al. (2019). Nature. 573(7775); 546-552).

Forma física

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitución

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Información legal

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable