NCI-H358

95111733, human lung (bronchoalveolar), Not specified

• Código UNSPSC: 41106514

Propiedades

• product name: NCI-H358, 95111733
• origen biológico: human lung (bronchoalveolar)
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian bronchioalveolar carcinoma

Descripción línea celular

NCI-H358 was isolated from a primary bronchioalveolar carcinoma of the lung from a Caucasian male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma of the lung (NSCLC) demonstrated the presence of granules characteristic of Clara cells. NCI-H358 do not express UDP-glucuronosyltransferases, but do express glutathione-S-transferase and phenol sulphotransferase. Expression of SP-A protein and RNA, the major surfactant-associated protein was detected. SP-B and SP-C RNA was not expressed. A complete homozygous deletion of the p53 gene and therefore a lack of p53 protein has been reported. A colony forming efficiency of 0.83% in soft agarose, and growth in serum-free media has been reported. The cells are tumourigenic in athymic nude mice, and exhibit a doubling time of 38 hours in RPMI 1640 medium. Since these cells are proficient in oxidation of xenobiotics but deficient in their conjugation with glucuronic acid they present a tool for analysing the role of glucuronic acid conjugation in the inactivation of chemicals in intact cells.

Aplicación

Test system for evaluating cytotoxicity and genotoxicity of chemicals to human lung

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12,13
D5S818: 10,12
D7S820: 10,11
THO1: 6
TPOX: 8,9
vWA: 17

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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