NCI-H322 Cell Line human

95111734, human lung (cervical node metastasis), Not specified

• Sinónimos: H322 Cells, NCI-322 Cells, NCI-H322T Cells
• Código UNSPSC: 41106514

Propiedades

• product name: NCI-H322 Cell Line human, 95111734
• origen biológico: human lung (cervical node metastasis)
• descripción: Human Caucasian bronchioalveolar carcinoma
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian bronchioalveolar carcinoma

Descripción línea celular

NCI-H322 was derived in 1981 from a primary bronchioalveolar carcinoma of the lung from a 52 year old male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma cell line demonstrated the presence of cytoplasmic structures characteristic of Clara cells. NCI-H322 do not express UDP-glucuronosyltransferase, but are reported to express glutathione-S-transferase and phenol sulfotransferase. Expression of protein and RNA of SP-A, the major surfactant-associated protein was detected. The cells produce tumours in athymic mice.

Aplicación

NCI-H322 has been used to study the preclinical antitumor efficacy of BAY 1129980, which is an anti-C4.4A (Ly6/PLAUR domain-containing protein 3 precursor-LYPD3) antibody–drug conjugate for the treatment of non–small cell lung cancer.

Test system for evaluating cytotoxicity and genotoxicity of chemicals to human lung

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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