HT29 Cell Line human

91072201, human colon, Epithelial

• Sinónimos: HT29 Cells
• Código UNSPSC: 41106514

Propiedades

• product name: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• origen biológico: human colon
• modo de crecimiento: Adherent
• cariotipo: 2n = 46, hypertriploid
• morfología: Epithelial
• productos: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian colon adenocarcinoma

Descripción línea celular

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

Aplicación

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

Medio de cultivo

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

Otras notas

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