RCE

95081046, rabbit eye, Epithelial-like

• Código UNSPSC: 41106514
• origen biológico: rabbit eye
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial-like
• productos: Not specified
• receptores: Not specified

Propiedades

• Nombre del producto: RCE, 95081046
• origen biológico: rabbit eye
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rabbit corneal epithelial cells, SV40 transformed

Descripción línea celular

The RCE cell line, also known as K′RCE, was established after infection of primary cultured rabbit (New Zealand albino) corneal epithelial cells with a recombinant SV40 vector. The cells exhibit a cobblestone-like appearance and stratification, including desmosome formation and microvilli development. Expression of keratin AE1 was always detectable whereas AE5 was expressed only at confluency.

Aplicación

Corneal epithelial research

Medio de cultivo

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μg/ml Insulin + 10ng/ml EGF + 0.1μg/ml Cholera toxin + 0.5% Dimethyl Sulphoxide (DMSO) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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