CAD cell line from mouse

8100805, mouse nerve, Neuronal

• Sinónimos: CAD Cell Line
• Código UNSPSC: 41106514

Propiedades

• product name: CAD cell line from mouse, 08100805
• origen biológico: mouse nerve
• descripción: Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Neuronal
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

Descripción línea celular

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

Aplicación

Study of neuronal differentiation

Medio de cultivo

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 10⁴ cells/cm² Cells should be dislodged by gentle pipetting; 5% CO₂; 37°C.

Otras notas

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