PG

95070629, fish ovary, Fibroblast-like

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: PG, 95070629
• origen biológico: fish ovary
• envase: tube of 5 μg 95070629-DNA-5UG; pkg of vial of cells 95070629-1VL
• modo de crecimiento: Adherent
• cariotipo: Aneuploid
• morfología: Fibroblast-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Fish Northern Pike ovaries

Descripción línea celular

The fish cell line PG has been initiated 1976 from ovarian tissue of 5 juvenile pike (Esox lucius). Cells grow at 4°C to 25°C with an optimum temperature of 20°C. PG cells are susceptible to various fish viruses including infectious pancreatic necosis viruses and pike fry rhabdovirus. The cell line has been mycoplasma eradicated at ECACC.

Aplicación

Fish Virology

Medio de cultivo

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week wit

Otras notas

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