EPC

93120820, fish skin, Epithelial

• Código UNSPSC: 41106514
• origen biológico: fish skin
• modo de crecimiento: Adherent
• cariotipo: 2n = 96
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified

Propiedades

• Nombre del producto: EPC, from fish skin, 93120820
• origen biológico: fish skin
• modo de crecimiento: Adherent
• cariotipo: 2n = 96
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice

Descripción

Origen línea celular

Fish epithelioma Fathead minnow Pimephales promelas

Descripción línea celular

This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support the growth of pancreatic necrosis virus and rhabdovirus.

Perfil del ADN

Not specified

Medio de cultivo

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO₂; 20 °C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Otras notas

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