AtT-20/D16v-F2

94050406, mouse brain, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: AtT-20/D16v-F2, 94050406
• origen biológico: mouse brain
• envase: tube of 5 μg 94050406-DNA-5UG; pkg of vial of cells 94050406-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse pituitary tumour

Descripción línea celular

This F2 subclone was developed from original AtT-20, an andrenocorticotropic hormone (ACTH) secreting cell line established from murine pituitary tumour. These cells are readily transfected using a standard calcium phosphate protocol.

Aplicación

AtT-20/D16v-F2 has been used to study the role of the G-protein Rasd1 in hypothalamic response to neuronal activation. It has also been used to study the effect of cAMP and glucocorticoid treatment on cAMP-responsive element binding protein 3 like 1 (CREB3L1).

Medio de cultivo

DMEM + 2mM Glutamine + 10% FBS.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:5 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cell clumps must be broken up to prevent cells growing in suspension.

Otras notas

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