NCTC clone 1469

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88111403, mouse liver, Epithelial

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse liver
• envase: tube of 5 μg 88111403-DNA-5UG; pkg of vial of cells 88111403-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse C3H/An liver

Descripción línea celular

Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

Aplicación

Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)

Medio de cultivo

NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO₂; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan

Otras notas

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