NB4 1A3

89121405, mouse nerve, Neuroblast

• Código UNSPSC: 41106514

Propiedades

• product name: NB4 1A3, 89121405
• origen biológico: mouse nerve
• modo de crecimiento: Adherent
• cariotipo: Unstable
• morfología: Neuroblast
• productos: Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse C-1300 Neuroblastoma

Descripción línea celular

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

Aplicación

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

Medio de cultivo

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Otras notas

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