HEPM

90120505, human palate epithelium. Established from palatal shelves.

• Código UNSPSC: 41106514

Propiedades

• product name: HEPM, 90120505
• origen biológico: human mouth (embryonic)
• modo de crecimiento: Adherent
• cariotipo: 2n = 46, diploid
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human embryonic palatal mesenchyme

Descripción línea celular

Established from embryonic palatal shelves. The cells are very sensitive to the stimulating effect of EGF with respect to proliferation, incorporation of thymidine and ornithine decarboxylase.

Aplicación

Proliferation studies

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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