CAR

89072611, fish fin, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: CAR, 89072611
• origen biológico: fish fin
• envase: tube of 5 μg 89072611-DNA-5UG; pkg of vial of cells 89072611-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Goldfish fin tissue

Descripción línea celular

The cells support the growth of grunt fin agent with CPE at 20°C and 25°C. Also susceptible to infectious pancreatic necrosis virus of trout at 28°C.

Aplicación

Growth factors and virus studies: Infectious pancreatic necrotic virus

Medio de cultivo

BME (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin; 5% CO₂; 25°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media ch

Otras notas

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