BF-2

87032603, fish trunk, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• product name: BF-2, 87032603
• origen biológico: fish trunk
• modo de crecimiento: Adherent
• cariotipo: 2n = 48
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Fish Bluegill fry caudal trunk

Descripción línea celular

Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15°C to 33°C. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.

Aplicación

Virus studies, targets for bacterial cytotoxins

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (pH7.4 - 7.5).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin/EDTA; 5% CO₂; 23°C. A replication capacity of up to 50 passages is expected. Fish cell lines detach easily during transit if the culture is too young. After resuscitation, split cells

Otras notas

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