DEM human cell line

IHW Number: 9007, 88052007

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human blood
• etnicidad: German
• género: male
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblastoid
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice

Descripción

Descripción general

Consanguineous: No

Homozygous: No

Descripción línea celular

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Perfil del ADN

A: 201
Cw: 602
B: 5701
HLA DRA: 101
HLA DRB1: 0401 1602
HLA DRB4: 101
HLA DRB5: 2
HLA DRB6: 202
HLA DQA1: 0102 03
HLA DQB1: 0502 0302
HLA DPA1: 1
HLA DPB1: 0301 0401

Perfil serológico

HLA A: 2
HLA A ief: 2.2
HLA C: 6
HLA B: 57
HLA B ief: 57.2
Bw4/bw6: 4
HLA DR: 4 16
HLA DR51: 51
HLA DR53: 53
HLA DQ: 5 8
HLA DP: 3 4

Medio de cultivo

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rutina de subcultivo

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Otras notas

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Cláusula de descargo de responsabilidad

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.