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Merck
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Key Documents

SLM-240

Sigma-Aldrich

RPMI-1640 complete medium

With ʟ-glutamine and 10% US origin FBS, liquid, sterile-filtered, suitable for cell cutlure

Sinónimos:

Low Bicarb Formulation, Roswell Park Memorial Institute 1640 medium

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About This Item

Código UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.71

product name

RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS, supplemented with 2mM L-Glutamine and 10% U.S. origin FBS

Nivel de calidad

300

formulario

liquid

técnicas

cell culture | mammalian: suitable

Descripción general

RPMI 1640 CompleteMedium is supplemented with 2mM L-Glutamine and 10% U.S.-origin fetal bovineserum (FBS). This sterile solution is convenient to culture a wide range ofcells that grow in suspension or as anchorage dependent. It can also be used infusion protocols and for growing hybrid cells. Roswell Park Memorial Institute(RPMI) 1640 medium can be used for culturing human normal and neoplasticleukocytes. It supports the growth of various cultured cells, like fresh humanlymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay if theformulation is properly supplemented.

Aplicación

RPMI 1640 CompleteMedium, with 2mM L-Glut and 10% FBS has been used to culture: Jurkatlymphocytes and human U937 monocyte-like cell line(1), HT-29 and SW480 (humancolon cancer cell line) cells(2), monocytes(3)

Calidad

pH= 7.2-7.5
Osmolality= 270-292 mOsm/kg
Endotoxin= ≤2 EU/mL
Mycoplasma= Negative
Sterility Tested= Negative for Growth
Appearance= Orange/Red Clear Liquid
Cell Line Tested= A2780 Human Ovarian Carcinoma

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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