BeWo Cell Line human

86082803, human placenta, Epithelial

• Sinónimos: Be-Wo Cells
• Código UNSPSC: 41106514

Propiedades

• product name: BeWo Cell Line human, 86082803
• origen biológico: human placenta
• descripción: Human choriocarcinoma
• modo de crecimiento: Adherent
• cariotipo: modal number =86 range 71-178
• morfología: Epithelial
• productos: Gonadotrophin, lactogen and steroid hormones
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human choriocarcinoma

Descripción línea celular

The first human, trophoblastic endocrine cell type to be maintained in continuous culture. It was initiated from a malignant gestational choriocarcinoma of the foetal placenta. The cells secrete placental hormones including chorionic gonadotrophin (hCG), polypeptide hormones, lactogen, estrogenic and progestational steroids, estrone, estradiol, estriol and progesterone. A high rate of hCG Synthesis has been shown. Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. Due to the high glycolytic activity the cells have to be sub-cultured or supplied with fresh media every 24-48 hours to prevent glucose exhaustion. BeWo has the same DNA profile as JEG3 (Sigma catalogue number 92120308); JEG3 was established by serial cloning of BeWo.

Aplicación

Hormone studies

Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. It has also been used to study placental transfer of highly purified non-dioxin-like polychlorinated biphenyls in BeWo cells.

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 16

Medio de cultivo

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C; medium change or subculture every 24-48 hours necessary.

Otras notas

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