A549 Cell Line human

from human lung(carcinoma), 86012804

• Sinónimos: A-549 Cells, A549 Cells, NCI-A549 Cells
• Código UNSPSC: 41106514

Propiedades

• origen biológico: human lung (carcinoma)
• modo de crecimiento: Adherent
• cariotipo: Hypotriploid
• morfología: Epithelial
• productos: Lecithin, High amounts of de-saturated fatty acids.
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian lung carcinoma

Descripción línea celular

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Aplicación

Enzymatic studies

Human A549 cell line has been used:

• to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
• to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
• to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Medio de cultivo

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or tTrypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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