CASP8C
Caspase 8 Assay Kit, Colorimetric
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.84
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Condiciones de envío
dry ice
Nivel de calidad
temp. de almacenamiento
−20°C
Información sobre el gen
human ... CASP8(841)
Descripción general
Caspases (cysteine-requiring aspartate proteases) belong to a highly conserved family of cysteine proteases with specificity for aspartic acid residues found in their substrates. Caspases play a central role in apoptosis. Inhibition of caspases prevents the cell death process. Currently, there are 13 caspases recognized in mammals. Caspases are classified as initiators and effectors. Caspase 8 belongs to the class of initiators. It possesses a prodomain at the terminal and contains the death effector domain which serves as protein-protein interaction motif for caspase 8. With respect to substrate specificity, caspase 8 is classified as a member of group III. It specifically recognizes the (I/L/V) EXD sequence on the substrate.
Aplicación
The Caspase 8 Colorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-p-Nitroaniline (Ac-IETD-pNA) by caspase-8 that results in the release of a p-Nitroaniline (p-NA) moiety. The p-NA is read at 405 nm. The concentration of the p-NA released can be calculated from a calibration curve prepared from p-NA standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
- purified caspase 8 for a positive control
- caspase 8 inhibitor
- p-NA fluorescent standard
Cantidad
The kit provides sufficient reagents for 100 tests of 100 μL in 96-well plates.
Solo componentes del kit
Referencia del producto
Descripción
- N-Acetyl-Ile-Glu-Thr-Asp-al, ≥97% (HPLC) 100 μL
- Acetyl-Ile-Glu-Thr-Asp-p-nitroaniline Substrate 100 μL
- 10X Assay Buffer 5 mL
- 5X Lysis Buffer 5 mL
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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