CASP3F
Caspase 3 Assay Kit, Fluorimetric
Sinónimos:
Caspase-3 Activity Fluorometric Kit
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About This Item
Productos recomendados
uso
sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)
Nivel de calidad
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Información sobre el gen
human ... CASP3(836)
Descripción general
The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).
Aplicación
The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.
Definición de unidad
One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.
Solo componentes del kit
Referencia del producto
Descripción
- Water (17-18 megaOhm) 125 mL
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
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