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B8931

Sigma-Aldrich

Magenta-Gal

reagent for selection of recombinant bacterial clones

Sinónimos:

Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside

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About This Item

Fórmula empírica (notación de Hill):
C14H15BrClNO6
Número de CAS:
93863-88-8
Peso molecular:
408.63
Número MDL:
MFCD02683897
Código UNSPSC:
12352200
ID de la sustancia en PubChem:
329773145
NACRES:
NA.85

grado

for molecular biology

Nivel de calidad

200

esterilidad

non-sterile

Línea del producto

BioReagent

Análisis

≥98.0% (HPLC)

formulario

powder

solubilidad

methanol: soluble

idoneidad

suitable for substrate for β-galactosidase

temp. de almacenamiento

−20°C

cadena SMILES

OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1

Clave InChI

CHRVKCMQIZYLNM-HTOAHKCRSA-N

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Descripción general

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Red-Gal is a chromogenic substrate for b-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Aplicación

Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein. ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.
Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.

Principio

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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