A9792

Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinónimos: Rabbit Anti-Chicken IgY (IgG) (whole molecule)−HRP
• Número MDL: MFCD00162225
• Código UNSPSC: 12352203
• NACRES: NA.46

Propiedades

• origen biológico: rabbit
• conjugado: peroxidase conjugate
• forma del anticuerpo: IgG fraction of antiserum
• tipo de anticuerpo: secondary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• reactividad de especies: chicken
• técnicas: direct ELISA: 1:30,000; dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate.; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody.
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.

Especificidad

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgY.

Inmunógeno

purified chicken IgY (IgG)

Aplicación

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used in immunohistology.

Avian proteins over expressed and purified from E. coli were analyzed by western blot using HRP-conjugated rabbit anti-chicken IgY as the secondary at 1:2000 dilution.

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for ELISA. The antibody can also be used for dot blot (1:160,000) and IHC (1:500) assays.

Acciones bioquímicas o fisiológicas

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.

IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Nota de preparación

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317,H334
• Consejos de prudencia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable