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F4137

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−Fluorescein isothiocyanate

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About This Item

Número MDL:
MFCD00162224
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

rabbit

conjugado

FITC conjugate

forma del anticuerpo

IgG fraction of antiserum

tipo de anticuerpo

secondary antibodies

clon

polyclonal

formulario

buffered aqueous solution

condiciones de almacenamiento

protect from light

técnicas

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
indirect immunofluorescence: 1:200

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .

Especificidad

Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration.

Inmunógeno

Chicken IgY (IgG)
Purified chicken IgY (IgG)

Aplicación

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used:
  • in indirect immunofluorescence
  • in western blotting
  • in immunocytochemistry

Acciones bioquímicas o fisiológicas

IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protection of chickens against infectious bronchitis by a recombinant fowlpox virus co-expressing IBV-S1 and chicken IFN gamma
Wang Y F, et al.
Vaccine, 27(50), 7046-7052 (2009)
Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage
Bar-Nur O, et al.
Nature Biotechnology, 33(7), 761-761 (2015)
Aymn T Abbas et al.
Vaccines, 8(4) (2020-11-04)
The Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in 2012 and causes severe and often fatal acute respiratory illness in humans. No approved prophylactic and therapeutic interventions are currently available. In this study, we have developed egg yolk antibodies
Ori Bar-Nur et al.
Nature biotechnology, 33(7), 761-768 (2015-06-23)
Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, has been reported to trigger transdifferentiation of fibroblasts into other cell types. Here we show that OKSM expression in mouse fibroblasts gives
G W Warr et al.
Immunology today, 16(8), 392-398 (1995-08-01)
IgY is the functional equivalent of IgG in birds, reptiles and amphibia, but many aspects of its biology are poorly understood. Recent studies have increased awareness of the genetics and functions of this molecule, and have revealed its position as
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