A7080
Amphiregulin human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Sinónimos:
AREG, CRDGF, SDGF, AR
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About This Item
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Nombre del producto
Amphiregulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
origen biológico
human
Nivel de calidad
recombinante
expressed in E. coli
Ensayo
≥97% (SDS-PAGE)
Formulario
lyophilized powder
potencia
10-100 ng/mL ED50/EC50
mol peso
~11 kDa
envase
pkg of 1 mg
condiciones de almacenamiento
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
técnicas
cell culture | mammalian: suitable
impurezas
endotoxin, tested
Nº de acceso UniProt
temp. de almacenamiento
−20°C
Información sobre el gen
human ... AREG(374)
Acciones bioquímicas o fisiológicas
Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.
Componentes
Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.
Precaución
This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.
Forma física
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.
Nota de preparación
It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.
Nota de análisis
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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