A6781
Acetate Kinase from Bacillus stearothermophilus
lyophilized powder, 400-1,200 units/mg solid
Sinónimos:
ATP:Acetate phosphotransferase, Acetate Kinase Bacillus stearothermophilus
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About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
32160410
Productos recomendados
origen biológico
bacterial (Bacillus stearothermophilus)
Nivel de calidad
Formulario
lyophilized powder
actividad específica
400-1,200 units/mg solid
temp. de almacenamiento
2-8°C
Aplicación
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. [32P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [32P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.
Acciones bioquímicas o fisiológicas
Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 % α-helix and 43 % unordered structure.
Involved in the metabolism of propanoate, pyruvate and taurine.
Definición de unidad
One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30 °C.
Forma física
Contains Tris-HCl buffer.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Met. Corr. 1
Código de clase de almacenamiento
8A - Combustible corrosive hazardous materials
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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H Nakajima et al.
Journal of biochemistry, 86(5), 1169-1177 (1979-11-01)
Fructose 1,6-bisphosphate (FBP) stimulates the reaction of Bacillus stearothermophilus acetate kinase (AK). FBP changes the reaction curve for ATP from a sigmoidal type to a Michaelis-Menten one. The binding of FBP to AK was studied by an equilibrium dialysis method
F J Grundy et al.
Journal of bacteriology, 175(22), 7348-7355 (1993-11-01)
The Bacillus subtilis gene encoding acetate kinase was identified on the basis of sequence similarity to the Escherichia coli ackA gene and to a second E. coli gene closely related to ackA. Insertional inactivation of this region of the B.
V Molle et al.
Molecular microbiology, 36(6), 1265-1278 (2000-08-10)
whiK was one of five new whi loci identified in a recent screen of NTG-induced whi mutants and was defined by three mutants, R273, R318 and R655. R273 and R318 produce long, tightly coiled aerial hyphae with frequent septation. In
H Nakajima et al.
Journal of biochemistry, 84(1), 193-203 (1978-07-01)
1. Acetate kinase [EC 2.7.2.1] from an thermophile, B. stearothermophilus, was purified and crystalized. 2. This enzyme was shown to be a tetramer of identical subunits which had a molecular weight of about 40,000. Amino acid analysis showed no SH
Smirla Ramos-Montañez et al.
Journal of bacteriology, 192(24), 6390-6400 (2010-10-19)
Acetyl phosphate (AcP) is a small-molecule metabolite that can act as a phosphoryl group donor for response regulators of two-component systems (TCSs). The serious human respiratory pathogen Streptococcus pneumoniae (pneumococcus) synthesizes AcP by the conventional pathway involving phosphotransacetylase and acetate
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