77332

Peroxidase from horseradish

lyophilized, powder, ~150 U/mg

• Sinónimos: (2R,3S,6S,7R,8R)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-dioxonan-7-yl 3-methylbutanoate, Antimycin A, Horseradish peroxidase
• Número de CAS: 9003-99-0
• Comisión internacional de enzimas: 1.11.1.7 (BRENDA, IUBMB)
• Número CE: 232-668-6
• Número MDL: MFCD00071339
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: horseradish
• Formulario: powder
• calidad: lyophilized
• actividad específica: ~150 U/mg
• mol peso: M_r ~40000
• solubilidad: 50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• cadena SMILES: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• Clave InChI: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Descripción

Descripción general

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.

Aplicación

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.

Peroxidase from horseradish has been used:

• for isolation of N-Linked glycans
• as a constituent of D-amino acid oxidase (DAAO) buffer
• to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)

Definición de unidad

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Otras notas

Use of chemically modified peroxidase in benzenecitation; Cross-linking of proteinscitation; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewatercitation

Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)