10108090001
Roche
Peroxidase (POD)
grade I, from horseradish
Sinónimos:
POD, peroxidase
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About This Item
Productos recomendados
origen biológico
horseradish
Nivel de calidad
Formulario
lyophilized (salt-free)
actividad específica
≥250 units/mg protein (At 25 °C with guaiacol and H2O2 as the substrates.)
250 U/mg
envase
pkg of 25,000 U
fabricante / nombre comercial
Roche
pH óptimo
6.0-6.5
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Descripción general
Donor:hydrogen-peroxide oxidoreductase
Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.
Aplicación
Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.
Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H2O2 produced from disaggregated slug cells.
Acciones bioquímicas o fisiológicas
Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.
Calidad
Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase
Purity number: approximately 3.0 (A403/A275)
Purity number: approximately 3.0 (A403/A275)
Nota de preparación
Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).
Reconstitución
Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1 - Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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