65497

TRIS acetate-EDTA Buffer solution

for Northern and Southern blotting, solution

• Código UNSPSC: 12161700

Propiedades

• grado: Molecular Biology; for molecular biology
• Línea del producto: BioReagent
• Formulario: solution
• impurezas: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 8.3±0.2
• densidad: 1.00 g/mL at 20 °C
• temp. de almacenamiento: room temp

Descripción

Descripción general

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This concentrate can be easily diluted to 0.5x before use (with molecular biology grade water).

Aplicación

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. Especially useful for dsDNA separations.

Componentes

The TAE liquid buffer is a 1x solution that contains 0.04M TRIS acetate and 0.001M EDTA in water.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves