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Merck

51146

Sigma-Aldrich

Atto 550 Protein Labeling Kit

BioReagent, suitable for fluorescence

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

Línea del producto

BioReagent

Nivel de calidad

fabricante / nombre comercial

ATTO-TEC GmbH

fluorescencia

λex 544 nm; λem 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

idoneidad

suitable for fluorescence

temp. de almacenamiento

2-8°C

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Descripción general

This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

Aplicación

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550 Protein Labeling Kit contains Atto 550 succinimidyl ester which enables conjugation of Atto 550 to the primary amino groups of the proteins and other molecules.

Información legal

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3


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Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Andrew H J Salmon et al.
Journal of the American Society of Nephrology : JASN, 23(8), 1339-1350 (2012-07-17)
Patients with albuminuria and CKD frequently have vascular dysfunction but the underlying mechanisms remain unclear. Because the endothelial surface layer, a meshwork of surface-bound and loosely adherent glycosaminoglycans and proteoglycans, modulates vascular function, its loss could contribute to both renal
Antibody transfection into neurons as a tool to study disease pathogenesis.
Douglas JN, Gardner LA, et al.
Journal of Visualized Experiments, 26, 4154-4154 (2012)
Sandeep Kumar Vashist
Analytical biochemistry, 421(1), 336-338 (2011-11-19)
A surface plasmon resonance (SPR)-based procedure was developed to determine the effect of antibody modifications on its biomolecular binding behavior. Mouse immunoglobulin G (IgG) was immobilized on a protein A-functionalized gold-coated SPR chip. Goat anti-mouse IgG and its various commercially
Michel Frigoli et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 15(33), 8319-8330 (2009-07-04)
Core-shell type dual fluorescent nanoparticles (NPs) in the 16 nm diameter range with a selective ligand (cyclam) attached to the surface and two fluorophores--9,10-diphenyl-anthracene (donor, D) and pyrromethene PM 567 (acceptor, A)--embedded within the polymer core were synthesized and their

Artículos

Sigma® offers protein labeling kits based on two types of fluorescent dyes, the Atto dyes and the Tracy dyes.

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