C0537

5-Carboxyfluorescein

≥99% purity (HPLC), powder

• Sinónimos: 5-FAM
• Fórmula empírica (notación de Hill): C₂₁H₁₂O₇
• Número de CAS: 76823-03-5
• Peso molecular: 376.32
• Beilstein: 57037
• Número MDL: MFCD00036874
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24892258
• NACRES: NA.47

Propiedades

• product name: 5-Carboxyfluorescein, ≥99% (HPLC)
• Nivel de calidad: 200
• Análisis: ≥99% (HPLC)
• formulario: powder
• solubilidad: 0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: 2-8°C
• cadena SMILES: OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• Clave InChI: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Descripción

Aplicación

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Acciones bioquímicas o fisiológicas

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)