63184

5-Maleimido-eosin

for fluorescence, ≥93% (HPLC)

• Sinónimos: EMA, Eosin-5-maleimide
• Fórmula empírica (notación de Hill): C₂₄H₉Br₄NO₇
• Número de CAS: 150322-02-4
• Peso molecular: 742.95
• Número MDL: MFCD07437936
• Código UNSPSC: 12352207
• ID de la sustancia en PubChem: 329759808
• NACRES: NA.32

Propiedades

• grado: for fluorescence
• Nivel de calidad: 100
• Ensayo: ≥93% (HPLC)
• fluorescencia: λ_ex 524 nm; λ_em 545 nm (after derivatization with 2-mercaptoethanol)
• temp. de almacenamiento: 2-8°C
• cadena SMILES: Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1Br
• InChI: 1S/C24H9Br4NO7/c25-13-6-11-21(17(27)19(13)32)35-22-12(7-14(26)20(33)18(22)28)24(11)10-2-1-8(5-9(10)23(34)36-24)29-15(30)3-4-16(29)31/h1-7,32-33H
• Clave InChI: YRCLWXRIEYQTAV-UHFFFAOYSA-N

Descripción

Descripción general

5-Maleimido-eosin, also known as EMA or Eosin-5-maleimide, is a fluorescent binding agent. The 5-Maleimido-eosin binding test measures the EMA-labeled cells reduced mean channel fluorescence (MCF) reading.

Aplicación

Eosin-5-maleimide has been used as a fluorescent probe for the human erythrocyte band 3 protein. It is widely used as a diagnostic probe for the laboratory detection of hereditary spherocytosis (HS) by flow cytometry. The eosin-5-maleimide binding test was done to analyze the effect of alectinib (first line of treatment) for anaplastic lymphoma kinase (ALK).

Otras notas

Triplet probe. Measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)