XTG360-RO
Roche
X-tremeGENE™ 360 Transfection Reagent
Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines
Sinónimos:
transfection reagent
About This Item
Productos recomendados
grado
for molecular biology
formulario
liquid (aqueous solution)
envase
pkg of 0.4 mL (08724105001)
pkg of 1.0 mL (08724121001)
pkg of 5 × 1.0 mL (08724156001)
fabricante / nombre comercial
Roche
técnicas
transfection: suitable
temp. de almacenamiento
−20°C
Descripción general
Aplicación
- Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2.
- Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools.
- Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex.
- Suitable for transient and stable transfection.
- Functions well in the presence or absence of serum.
Características y beneficios
- Peak performance in a broad range of cell types
- Suitable for multiple applications
- Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components
Calidad
Forma física
Otras notas
Información legal
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Flam. Liq. 2
Código de clase de almacenamiento
3 - Flammable liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
61.7 °F
Punto de inflamabilidad (°C)
16.5 °C
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
MicroRNAs (miRNAs) are an endogenous class of RNA molecules comprising 19-25 nucleotides that regulate the suitability or transflational efficiency of mRNAs.
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.
Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.
Protocolos
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.
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