CAS9GFPPRO
Cas9-GFP Protein
from Streptococcus pyogenes, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS
Sinónimos:
Cas9-EGFP, SpCas9-EGFP, SpCas9-GFP
About This Item
Productos recomendados
recombinante
expressed in E. coli
Ensayo
≥90% (SDS-PAGE)
Formulario
lyophilized powder
envase
pkg of 1 kit (3 components)
gen reportero
GFP
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Descripción general
An N-terminally fused enhanced green fluorescent protein with an excitation peak at 488 nm and emission peak at 509 nm allows for visualization of transfected RNP complex in addition to utility in flow cytometry applications. The protein also contains three varied nuclear localization sequences postioned for optimal activity.
Aplicación
- Functional Genomics
- Target Validation
- Genome Editing
- Fluorescence Microscopy
- Flow Cytometry
Características y beneficios
- Highly specific
- Highly active
- Ready-to-inject/transfect
Envase
- pkg of 50 μg( ≥260 pmol )
- pkg of 250 μg( ≥1300 pmol )
Componentes
- one vial of lyophilzed Cas9-GFP recombinant protein
- one vial containing 1 mL of 1× dilution buffer
- one vial containing 1 mL of nuclease-free water with glycerol
Principio
Reconstitución
Otras notas
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Información legal
Código de clase de almacenamiento
11 - Combustible Solids
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Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
Watch Cas9-GFP proteins work inside your cells and deliver the powerful nuclease editing you have come to trust and expect. MISSION™ SpCas9-GFP and eSpCas9-GFP proteins are multi-functional enzymes with high on-target cleavage efficiency fused to Enhanced Green Fluorescent Protein (EGFP) for real-time observation of Cas9 delivery and clearance from cells.
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