Saltar al contenido
Merck

EXPEXTKB

Roche

KAPA Express Extract

Sinónimos:

PCR, PCR extraction

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
41106300
NACRES:
NA.55

descripción

for extraction only

Nivel de calidad

uso

sufficient for 100 reactions
sufficient for 250 reactions
sufficient for 50 reactions
sufficient for 500 reactions

caducidad

≤12 mo.

envase

pkg of 100 U (KK7100)
pkg of 1000 U (KK7103)
pkg of 200 U (KK7101)
pkg of 500 U (KK7102)

fabricante / nombre comercial

Roche

técnicas

DNA extraction: suitable
PCR: suitable

temp. de almacenamiento

−20°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Descripción general

KAPA Express Extract is a novel thermostable protease and buffer system that allows for the extraction of polymerase chain reaction (PCR)-ready DNA in as little as 15 minutes. DNA extractions are conveniently performed in a single-tube thereby greatly reducing the risk of sample loss and contamination.
The combination of KAPA Express Extract and KAPA2G Robust HotStart® ReadyMix provides a high performance solution for rapid DNA extraction and consistent downstream amplification.

Aplicación

KAPA Express Extract is ideally suited for the extraction of polymerase chain reaction (PCR)-ready DNA from, but not limited to, the following sample types:
  • Human tissue (formalin-fixed paraffin-embedded (FFPE) samples; blood collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples; biopsies)
  • Animal samples (ear punches; tail clippings; hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)
  • Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples; embryo)
  • Insects (crushed)
  • Bird feathers (calamus fragments).
  • Nosema species

Características y beneficios

Key Features:
  • Rapid extraction protocol: PCR-ready DNA in 15 minutes.
  • Versatility: single kit optimized for successful extraction from a variety of tissue types, including buccal swabs, hair follicles, fish tissue, and bird feathers.
  • Single-tube reaction minimizes risk of contamination.
  • Produces high amounts of genomic DNA of good quality.
  • DNA extracted with KAPA Express Extract is compatible with any PCR system.

Increased PCR success from formalin-fixed paraffin-embedded (FFPE) samples or tissues:
  • Rapid DNA extraction and consistent downstream amplification of challenging sample types.

Routine DNA extraction from blood sample types:
  • Efficient extraction from “Guthrie”cards, FTA Elute cards, and FTA cards.

Quick Notes:
  • Extract PCR-ready DNA from different tissue types in a quick and simple, single-tube protocol (15–20 min), without the need for hazardous chemicals or multiple washing steps.
  • Significantly reduced turnaround times and risk of sample loss and contamination.
  • Use 1 μL DNA extract per 25 μL PCR. Depending on the sample type, the extract may have to be diluted prior to PCR.
  • One extract typically yields a sufficient amount of template for 50–500 PCRs.
  • DNA extracts may be diluted in TE Buffer (1:5) or 10 mM Tris-HCl pH 8–8.5 (1:5–1:10) for long-term storage at -20°C. Extracts are stable at -20°C for ≥6 months.
  • KAPA2G Robust HotStart® ReadyMix is recommended for consistent and reliable endpoint amplification of DNA extracts generated with KAPA Express Extract.
  • No quantification of DNA required prior to PCR.

Calidad

All components contained with KAPA Express Extract Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activities, and meet strict requirements with respect to DNA contamination.

Nota de preparación

Handling:
KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.

Otras notas

For Research Use Only. Not for use in diagnostic procedures.

Información legal

FTA is a trademark of Qiagen Group
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Solo componentes del kit

Referencia del producto
Descripción

  • KAPA Express Extract Enzyme 1 U/µL

  • 10X KAPA Express Extract Buffer

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Characteristics of Nosema ceranae infection in Serbian honey bee colonies
Stevanovic J, et al.
Apidologie, 44 (2013)
BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment
Boel A, et al.
Scientific reports (2016)
The Validity of Real Time PCR and in Situ Hybridization in the Detection of JCV in Colonic Biopsies of Patient with Colorectal Cancer
Aldrubi FI, et al.
International Journal of Current Microbiology and Applied Sciences, 4(12), 107-120 (2015)
Molecular Study of Some Virulence Genes in Biotype Diversity of Methicillin Resistance Staphylococcus aureus isolated from Handling Carrier and Bovine Mastitis.
Hanon B M.
International Journal of Scientific Research, 6(2), 2319-7064 (2017)
Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis.
Gajic B, et al.
Experimental & Applied Acarology, 61(1), 97-105 (2013)

Artículos

An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico