DCTP-RO

dCTP

99% (dNTP), <0.9% (dNDP), solution, 100 mM

• Sinónimos: 2′-Deoxycytidine 5′-triphosphate disodium salt, dCTP-Na₂
• Fórmula empírica (notación de Hill): C₉H₁₄N₃Na₂O₁₃P₃
• Número de CAS: 102783-51-7
• Peso molecular: 511.12
• Número MDL: MFCD00084683
• Código UNSPSC: 41106305
• ID de la sustancia en PubChem: 329798916

Propiedades

• descripción: PCR Grade, sodium salt
• Nivel de calidad: 100
• Análisis: 99% (dNTP)
• formulario: solution
• uso: sufficient for 125,000 standard reactions (03732690001); sufficient for 31,250 standard reactions (11969021001); sufficient for 6,250 standard reactions (11934520001)
• envase: pkg of 1,250 μL (11969021001 [100 mM]); pkg of 4 × 1,250 μL (03732690001 [4x100 mM]); pkg of 250 μL (11934520001 [100 mM])
• fabricante / nombre comercial: Roche
• concentración: <0.9% (dNDP); 100 mM
• temp. de almacenamiento: −20°C
• cadena SMILES: [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
• InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
• Clave InChI: ABWVCNMFYVEBIB-CDNBRZBRSA-L

Descripción

Descripción general

dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Aplicación

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Calidad

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Forma física

solution of dCTP in water (pH 8.3).

Nota de preparación

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Otras notas

For general laboratory use.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash