DGTP-RO

dGTP

PCR Grade, sodium salt

• Sinónimos: 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, dGTP-Na₃
• Fórmula empírica (notación de Hill): C₁₀H₁₃N₅Na₃O₁₃P₃
• Número de CAS: 93919-41-6
• Peso molecular: 573.13
• Número MDL: MFCD00080337
• Código UNSPSC: 12352302
• ID de la sustancia en PubChem: 329798919

Propiedades

• Nivel de calidad: 100
• Análisis: 99% (dNTP)
• formulario: solution
• uso: sufficient for 125,000 standard reactions (03732703001); sufficient for 31,250 standard reactions (11969030001); sufficient for 6,250 standard reactions (11934538001)
• envase: pkg of 1,250 μL (11969030001 [100 mM]); pkg of 4 × 1,240 μL (03732703001 [4x100 mM]); pkg of 250 μL (11934538001 [100 mM])
• fabricante / nombre comercial: Roche
• concentración: <0.9% (dNDP); 100 mM
• temp. de almacenamiento: −20°C
• cadena SMILES: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
• InChI: 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
• Clave InChI: IWGGLKOTEOCWQP-BIHLCPNHSA-K

Descripción

Descripción general

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Aplicación

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

Calidad

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Forma física

solution of dGTP in water (pH 8.3).

Nota de preparación

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Otras notas

For general laboratory use.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash