MABN627

Anti-SDHB Antibody, clone 10C5.1

clone 10C5.1, from mouse

• Sinónimos: Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, SDHB, Iron-sulfur subunit of complex II, Ip
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 10C5.1, monoclonal
• reactividad de especies: human, mouse
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_002991
• Nº de acceso UniProt: P21912
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SDHB(6390)

Descripción

Descripción general

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC 1.3.5.1; UniProt P21912; also known as Ip, Iron-sulfur subunit of complex II) is encoded by the SDHB (also known as IP, SDH, CWS2, PGL4, SDH1, SDHIP) gene (Gene ID 6390) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential cancer markers and targets for treatment. Succinate dehydrogenase (SDH), also known as mitochondrial respiratory Complex II, is a holoenzyme consisting of four essential subunits, SDHA (a flavoprotein), SDHB (iron-sulfur protein), and two membrane anchor units SDHC and SDHD. SDH assembly requires two factors, SDHAF1 and SDHAF2, while its function is influenced by the deacetylase activity of SIRT3. SDH is the only enzyme complex known to participate in both the TCA cycle and electron transport chain (ETC.). SDH catalyzes the oxidation of succinate in the TCA to form fumarate, a reaction that is coupled to the reduction of ubiquinone to ubiquinol in the ETC. SDH loss-of-function mutations have been linked to pheochromocytoma (PCC), paraganglioma (PGL), gastrointestinal stromal tumor (GIST), renal cell carcinoma, and ovarian cancer.

Inmunógeno

Epitope: C-terminal half

GST-tagged recombinant protein corresponding to the C-terminal half of human SDHB.

Aplicación

Anti-SDHB Antibody, clone 10C5.1 is an antibody against SDHB for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SDHB in 10 µg of Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SDHB in human heart, human small intestine, and mouse kidney tissue.

Calidad

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SDHB in 10 µg of HEK293 cell lysate.

Descripción de destino

~30 kDa observed

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable