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SAB5900202

Sigma-Aldrich

Alpaca Anti-PD-L1, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

Sinónimos:

B7-H, B7H1, PD-L1, PDCD1LG1, PDL1

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

alpaca

Nivel de calidad

recombinante

expressed in E. coli

conjugado

unconjugated

forma del anticuerpo

affinity purified VHH antibody

clon

PL03, monoclonal

Ensayo

>95% (SDS-PAGE)

Formulario

lyophilized solid

mol peso

14.4 kDa
~ 15-18 kDa (SDS PAGE under reducing condition)

purificado por

immobilized metal affinity chromatography (IMAC)

reactividad de especies

human, (Cynomolgus macaques)

concentración

1 mg/mL

técnicas

ELISA: 1:10000-1:100000
flow cytometry: 1:50-1:100
immunofluorescence: 1:200-1:800
immunohistochemistry: 1:200-1:800
western blot: 1:1000-1:3000

color

colorless

pH

( in PBS, pH 7.4)

isotipo

VHH

Nº de acceso Protein ID

Nº de acceso UniProt

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CD274(29126)

Descripción general

PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis.Alpaca derived anti-PD-L1 VHH single domain antibody (molecular weight: 14.4kDa), expressed in E. coli under conditions free from animal derived components. It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. The small size and monovalency makes it a superior tool in microsopy. These single-domain antibodies offer superior chemical (wide variety of pH, urea, etc) and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

Especificidad

Human Programmed Death-Ligand 1 (PD-L1)

Inmunógeno

Recombinant human PD-L1 (Phe19-Tyr134) protein

Aplicación

ELISA, WB, FC, IHC, IF

Envase

50 µg per vial / 100 µg per vial

Forma física

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.5

Reconstitución

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Almacenamiento y estabilidad

Stable for 1 year at 4°C from date of receipt.

Otras notas

Storage at 4°C; Do not freeze.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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