MABN1580

Anti-beta-Arrestin-2 Antibody, clone 5E12.1

clone 5E12.1, from mouse

• Sinónimos: Arrestin beta-2, beta-Arrestin-2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 5E12.1, monoclonal
• reactividad de especies: human, rat
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001244257
• Nº de acceso UniProt: P32121
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ARRB2(409)

Descripción

Descripción general

Beta-arrestin-2 (UniProt P32121; also known as Arrestin beta-2) is encoded by the ARRB2 (also known as ARB2, ARR2, BARR2) gene (Gene ID 409) in human. β-Arrestin 1 and 2 are cytosolic adapter proteins originally identified as negative regulators of G protein-coupled receptor (GPCR) signaling through receptor desensitization and internalization. It is now known that these multifunctional scaffold proteins also elicit G-protein-independent signalling pathways by linking the activated GPCRs to multiple signaling molecules, such as ERK1/2, Akt, and Src kinases. In addtion to GPCRs, β-arrestins are also involved in receptor tyrosine kinase (RTK) signalling pathways, and β-arrestin-2 is reported to link Src and Akt to insulin receptor (IR) to ensure a full Akt activation. Although β-arrestin-1 and β-arrestin-2 share a high degree of structural homology, they do display specific functions in several physiological processes. β-arrestins can adopt multiple conformations to display distinct sets of interacting domains. Recruitment of β-arrestins to a specific GPCR results in the formation of distinct β-arrestin scaffolds, allowing a high level of specificity for a given agonist.

Especificidad

Expected to react with spliced isoforms 1, 4, and 5 reported by UniProt (P32121). Reactivity toward spliced isoforms 2 & 3 has not been determined.

Inmunógeno

Epitope: C-terminal region.

KLH-conjugated linear peptide corresponding to a sequence in the C-terminal region of human beta-Arrestin-2.

Aplicación

Anti-beta-Arrestin-2 Antibody, clone 5E12.1 is an antibody against beta-Arrestin-2 for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected beta-Arrestin-2 in rat testis and human cerebral cortex & liver tissues.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Calidad

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected beta-Arrestin-2 in 200 µg of HeLa cell lysate.

Descripción de destino

~52 kDa observed. 46.11 kDa (isoform 1), 47.27 kDa (isoform 2), 45.56 kDa (isoform 3), 48.02 kDa (isoform 4), and 4439 kDa (isoform 5) calculated.

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable