MABF3178

Anti-IL-22 Antibody, clone 9F2.2.4

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies, primary antibodies
• clon: 9F2.2.4, monoclonal
• mol peso: calculated mol wt 20.01 kDa; observed mol wt ~17 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100 μg
• técnicas: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_065386.1?
• Nº de acceso UniProt: Q9GZX6
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... IL22(50616)

Descripción

Descripción general

Interleukin-22 (UniProt: Q9GZX6; also known as IL-22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-TIF) is encoded by the IL22 (also known as ILTIF, ZCYTO18, UNQ3099/PRO10096) gene (Gene ID: 50616) in human. IL-22 is an -helical cytokine that contributes to the inflammatory response in vivo. It is a secretory protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce the mature protein. It is immediately produced to initiate immune response against several tissue impairments. It is considered as a fundamental mediator in inflammation, mucous production, protective role against pathogens, wound healing, and tissue regeneration. It is produced by several populations of immune cells, including macrophages, β T cells classes Th1, Th22, and Th17 cells at the site of inflammation. It is shown to up-regulate a number of mucus associated genes, such as MUC1, MUC3, MUC10, and MUC13 in colonic and tracheal epithelial cells. Along with IL-17A, it also produces pulmonary mucus as a control against Gram negative pathogens. Its biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein (IL-22BP). IL-22 is shown to have a very high affinity for binding to IL-22BP, which acts as a natural antagonist of IL-22. IL-22BP binding occurs at specific sites on IL-22 that are also utilized by IL-22R1. (Ref.: Arshad, T., et al. (2020). Front. Immunol. 11; 2148).

Especificidad

Clone 9F2.2.4 is a mouse monoclonal antibody that detects human IL-22.

Inmunógeno

A full-length mature human IL-22 (without the signal peptide).

Aplicación

Quality Control Testing

Evaluated by Western Blotting with recombinant human IL-22.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant human IL-22.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-22 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-22, clone 9F2.2.4, Cat. No. MABF3178, is a mouse monoclonal antibody that detects human IL-22 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable