MABC1564

Anti-Pin1 Antibody, clone 5B4.1

clone 5B4.1, from mouse

• Sinónimos: Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC: 5.2.1.8, Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, Rotamase Pin1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 5B4.1, monoclonal
• reactividad de especies: human, rat
• envase: antibody small pack of 25 μg
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_006212
• Nº de acceso UniProt: Q13526
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PIN1(5300)

Descripción

Descripción general

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (UniProt: Q13526; also known as EC: 5.2.1.8, Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, Rotamase Pin1) is encoded by the PIN1 gene (Gene ID: 5300) in human. PPIase binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. Isomerization of phosphorylated Ser or Thr residues may alter protein confirmation and, subsequently, modify activity. By inducing conformational changes in a subset of phosphorylated proteins, it acts as a molecular switch in multiple cellular processes. PPIase is mainly localized in the nucleus, but phosphorylation at Serine 71 by death-associated protein kinase 1 (DAPK1) results in inhibition of its nuclear localization and its catalytic activity. It contains a WW domain (aa 5-39) and a peptidylprolyl isomerase C (PpiC) domain (aa 52-163). WW domain is shown to be essential for the interaction with STIL and KIF20B. PPIase displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. PPIase is reported to regulate mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. It is overexpressed in many human breast cancers, and has been shown to modify numerous proteins including p53, NF-kB, c-Jun, cyclin D1, and beta-catenin.

Especificidad

Clone 5B4.1 detects Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) in human and rat cells. It targets an epitope within 92 amino acids from the C-terminal half.

Inmunógeno

GST/His-tagged recombinant fragment corresponding to 92 amino acids from the C-terminal half of human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1.

Aplicación

Anti-Pin1, clone 5B4.1, Cat. No. MABC1564, is a mouse monoclonal antibody that detects Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Pin1 in human cerebral cortex tissue sections.

Research Category
Apoptosis & Cancer

Calidad

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Pin1 in 10 µg of PC-12 cell lysate.

Descripción de destino

~18 kDa observed; 18.24 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash