MAB427-C

Anti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free

clone 1A, from mouse

• Sinónimos: Voltage-dependent L-type calcium channel subunit alpha-1S, Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle, Voltage-gated calcium channel subunit alpha Cav1.1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1A, monoclonal
• reactividad de especies: plant, human, rabbit, mouse
• técnicas: immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001095190
• Nº de acceso UniProt: P07293
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PPP1R15A(23645)

Descripción

Descripción general

Voltage-dependent L-type calcium channel subunit alpha-1S (UniProt P07293; also known as Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle, Voltage-gated calcium channel subunit alpha Cav1.1) is encoded by the CACNA1S (also known as CACH1, CACN1, CACNL1A3) gene (Gene ID 100009585) in Oryctolagus cuniculus (rabbit) species. Voltage-dependent calcium channels (VDCC) are found in the membrane of excitable cells (e.g., muscle, glial cells, neurons) and exhibit 1000-fold greater permeability to calcium than to sodium under normal physiological conditions. There exist five types of VDCCs, including four high voltage-activated channels (Neural- or N-type channel, Residual- or R-type channel, Purkinje- or P/Q-type channel, and the dihydropyridine-sensitive Long-lasting or L-type channels) and the low voltage-activated Transient or T-type calcium channels. VDCCs exist as a complex of different subunits (α1, α2δ, β1-4, and γ), with α1 being the the ion-conducting pore-forming subunit that contains voltage-sensing machinery and the drug/toxin-binding sites. There exist ten genes coding for different α1 subunits, four of which code for L-type alpha-1 subunits (alpha-1A/Cav2.1/CACNA1A, alpha-1C/Cav1.2/CACNA1C, alpha-1D/Cav1.3/CACNA1D, and alpha-1S/Cav1.1/CACNA1S). The dihydropyridine-sensitive L-type channels are responsible for excitation-contraction coupling of skeletal, smooth, and cardiac muscle and for hormone secretion in endocrine cells. The α1S subunit (Cav1.1) contains the characteristic four homologous I–IV domains (a.a. 38-337, 418-664, 786-1068, 1105-1384) with six transmembrane α-helices each, having both its N- and C-terminal ends exposed intracellularly (a.a. 1-51, 1382-1873).

Especificidad

Clone 1A targets the extracellular region of voltage-dependent L-type calcium channel subunit alpha-1S or Cav1.1 encoded by the CACNA1S gene.

Inmunógeno

Dihydropyridine-binding complex isolated from rabbit muscle t-tubules.

Epitope: Extracellular domain.

Aplicación

Anti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free is an antibody against Cav1.1 alpha1s for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence, Immunoprecipitation.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Calidad

Evaluated by Western Blotting in Jurkat membrane extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cav1.1 alpha1s in 10 µg of Jurkat membrane extract.

Descripción de destino

~212 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable