MAB2226

Anti-Piccolo Antibody, clone 4G3.3

clone 4G3.3, from mouse

• Sinónimos: presynaptic cytomatrix protein, Aczonin
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: 4G3.3, monoclonal
• application: IHC; WB
• reactividad de especies: mouse
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• citations: 1

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 4G3.3, monoclonal
• reactividad de especies: mouse
• reactividad de especies (predicha por homología): human (based on 82% homology), rat (92% homology)
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgMκ
• Nº de acceso NCBI: NP_001104266
• Nº de acceso UniProt: Q9Y6V0
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PCLO(27445) ; mouse ... Pclo(26875)

Descripción

Descripción general

Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].

Especificidad

This antibody recognizes piccolo.

Inmunógeno

GST-tagged recombinant protein

Aplicación

Immunohistochemistry (Paraffin):
Representative images from a previous lot.
Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.

Calidad

Routinely evaluated by western blot on rat brain lysate.

Descripción de destino

547 kDa

Forma física

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Rat brain lysate, mouse cerebellum

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable