CC101

Human Integrin αVβ3 Protein, Triton X-100 Formulation

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Propiedades

• origen biológico: human
• Nivel de calidad: 100
• Ensayo: >95% (electrophoresis)
• Formulario: liquid
• fabricante / nombre comercial: Chemicon^®
• concentración: 200 μg/mL
• Nº de acceso NCBI: NM_002210.2
• Nº de acceso UniProt: P06756
• Información sobre el gen: human ... ITGAV(3685)

Descripción

Descripción general

Integrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaVbeta3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described [Belkin, 1990].



Two main protein bands corresponding to alphaV (145 kDa) and beta3 (92 kDa) subunits are seen in silver stained polyacrylamide gels under non-reducing conditions. Minor protein band (170 kDa) corresponds to beta3 subunit disulfide dimer.

Aplicación

Electrophoresis and Immunoblotting control. Also useful for in vitro binding

experiments with metalloproteinase MMP-2 .

Forma física

Purified protein in 20 mM Tris-HCI, pH 7.5, 150 mM NaCI, 2 mM MgCI2, 0.2% Triton X-100. No preservatives.

Almacenamiento y estabilidad

Maintain at -70°C in undiluted aliquots. Avoid repeated freeze/ thaw cycles.

Nota de análisis

7.5% SDS-PAGE by the method of Laemmli gives two major protein bands, corresponding to alpha V subunit (145 kDa) and beta 3 subunit (92 kDa) when visualized by silver stain (2-2.5 mg protein / lane nonreduced).A minor protein band (170 kDa) corresponds to the beta 3 dimer, similar to an occurrence of the beta 1 dimer upon purification of beta 1 integrins [Belkin et al., 1996].In some cases a beta 3 trimer is visible at 260 kDa.After reduction, with protein quantities of 1.5 - 4μg protein / lane, three major protein bands are apparent, corresponding to beta 3 integrin subunit (110 kDa), heavy alpha V chain (125 kDa) and light alpha V chain (25 kDa).A minor band at 200 kDa region is presumed to be the reduced beta 3 dimer, consistent with previous publications describing a 230 kDa beta 1 reduced dimer for beta 1 integrin purifications described previously.[Belkin et al.,1996].Faint bands at 80 kDa and 55 kDa correspond to minor degradation products of beta 3.CONTAMINANTS: Two faint bands identified at 210 and 220 kDa, which may correspond to the alpha V dimer.

Specific Activity: Functional activity confirmed by interaction of integrin alphaV/beta3 with vitronectin, von Willebrand Factor, fibrinogen, and fibronectin in RGD-dependent ELISA testing.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable